Enzyme-linked Immunosorbent Assay:Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, NY, USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat N° 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat N° 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat N∘ 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, PA, USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No. 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No. 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No. 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: IGF-1R antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: Briefly, 96-well ELISA plates (Costar 3690. .. Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa. USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (COSTAR® 3690, CORNING®, NY, USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D SYSTEMS®, cat No. 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D SYSTEMS®, cat No. 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D SYSTEMS®, cat No. 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% TWEEN® 20 (Polyethylene glycol sorbitan monolaurate) (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (# UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: IGF-1R antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No. 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No. 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No. 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (# UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Composition for the treatment of IGF-1R expressing cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat N° 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat N° 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat N° 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Incubation:Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, NY, USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat N° 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat N° 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat N∘ 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, PA, USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No. 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No. 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No. 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: IGF-1R antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: Briefly, 96-well ELISA plates (Costar 3690. .. Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa. USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (COSTAR® 3690, CORNING®, NY, USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D SYSTEMS®, cat No. 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D SYSTEMS®, cat No. 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D SYSTEMS®, cat No. 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% TWEEN® 20 (Polyethylene glycol sorbitan monolaurate) (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (# UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: IGF-1R antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No. 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No. 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No. 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (# UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Composition for the treatment of IGF-1R expressing cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat N° 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat N° 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat N° 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
Article Title: Antibody-drug-conjugate and its use for the treatment of cancer
Article Snippet: .. Briefly, 96-well ELISA plates (Costar 3690, Corning, N.Y., USA) were coated 100 μl/well of either the rhIGF-1R protein (R&D Systems, cat No 391-GR) at 0.6 μg/ml or rmIGF-1R protein (R&D Systems, cat No 6630-GR/Fc) at 1 μg/ml or rhIR protein (R&D Systems, cat No 1544-IR/CF) at 1 μg/ml in PBS overnight at 4° C. The plates were then blocked with PBS containing 0.5% gelatin (#22151, Serva Electrophoresis GmbH, Heidelberg, Germany) for 2 h at 37° C. Once the saturation buffer discarded by flicking plates, 100 μl of each supernatant dilution were added to each well (either undiluted hybridoma supernatant either supernatant serial dilutions) and incubated for 1 h at 37° C. After three washes, 100 μl horseradish peroxidase-conjugated polyclonal goat anti-mouse IgG (#115-035-164, Jackson Immuno-Research Laboratories, Inc., West Grove, Pa., USA) was added at a 1/5000 dilution in PBS containing 0.1% gelatin and 0.05% Tween 20 (w:w) for 1 h at 37° C. Then, ELISA plates were washed 3-times and the TMB (#UP664782, Uptima, Interchim, France) substrate is added. ..
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